Metabolic Labeling (SILAC)
Stable Isotope Labeling of Amino Acids in Cell Culture is a popular and simple MS technique using heavy amino acids to metabolically label the proteome of cultured cells in vivo.
UK distributor for Cambridge Isotope Laboratories
Heavy isotope labeling for mass spectrometry workflows
Heavy stable isotopes are routinely employed to generate precise and accurate quantitative proteomic data by comparing labeled heavy peptides with light peptides.
Stable Isotope Labeling of Amino Acids in Cell Culture is a popular and simple MS technique using heavy amino acids to metabolically label the proteome of cultured cells in vivo.
SILAM is a labeling method that employs heavy amino acids or 15N to metabolically label rodent tissues in vivo.
Synthesised peptides with or without heavy stable isotopes are invaluable for targeted single reaction monitoring assays and absolute MS quantitation; CIL offers amino acids and resin for synthesising peptides in your laboratory.
Chemical tagging refers to labeling proteins or peptides in vitro, an inexpensive method advantageous over metabolic labeling due to its ability to label human samples and quantify more than two samples in one MS experiment.
One of the simplest methods of labeling peptides in vitro is the incorporation of 18O during proteolytic digestion.
Expression of large amounts of unlabeled and labeled proteins in heterologous systems is an invaluable tool; CIL offers a variety of media for protein expression and kits for in vitro translation.
CIL offers PeptiQuant™ kits from MRM Proteomics Inc, including Monthly, Daily and Workflow Performance Kits, used to assess and track LC-MS platform performance in a proteomic workflow.
Don't hesitate to contact us if you have any questions or would like to discuss your requirements.